| Ontime Expedite LLC | 4045472 | 16 | 17 | Interstate |
| Owl INC | 1698514 | 15 | 5 | Interstate |
| Heavyweight Express INC | 4209209 | 10 | 5 | Interstate |
| Da & V Telecom Services LLC | 4476378 | 10 | 5 | Intrastate |
| J Arthur Services Corp | 3718320 | 8 | 8 | InterstateHazmat |
| Ag Auto Trans Corp | 4130730 | 8 | 4 | Interstate |
| Breakwater Construction Group LLC | 3240283 | 6 | 6 | Intrastate |
| Huselton & CO INC | 3301681 | 6 | 6 | Intrastate |
| S & B Hotshots LLC | 3060779 | 5 | 5 | Interstate |
| Trust B&b Express INC | 3098901 | 5 | 5 | Interstate |
| Koala Insulation Of Jacksonville | 3512520 | 5 | 6 | Intrastate |
| Sicher Trading LLC | 3806305 | 5 | 3 | Interstate |
| Astars Motors INC | 4359828 | 5 | 5 | Interstate |
| Republic Freight INC | 2490703 | 3 | 3 | Interstate |
| First Coast Mulch | 2832950 | 3 | 4 | Intrastate |
| Eb Freight LLC | 2992296 | 3 | 4 | Interstate |
| Sega Motors INC | 3163709 | 3 | 4 | InterstateHazmat |
| Digworx LLC | 3353420 | 3 | 3 | Intrastate |
| Concrete Staining INC | 3678634 | 3 | 3 | Interstate |
| Emperador Transportation LLC | 3710465 | 3 | 3 | Interstate |
| Kathi Trucking INC | 4118289 | 3 | 3 | Interstate |
| Cdit Transport LLC | 4216637 | 3 | 4 | Intrastate |
| Vipmar Construction Services LLC | 4334357 | 3 | 1 | Intrastate |
| Mark 13 General Services LLC | 4544391 | 3 | 6 | Interstate |
| The Academy At Julington Creek | 2477440 | 2 | 2 | Intrastate |
| Dinsmore Towing & Recovery | 3031597 | 2 | 2 | Intrastate |
| Dcv Logistics INC | 3447958 | 2 | 2 | Interstate |
| Obi Express | 3563810 | 2 | 2 | Interstate |
| United Wheels INC | 3575365 | 2 | 2 | Interstate |
| Intelligent Trucking INC | 3832630 | 2 | 2 | Interstate |
| Quill's Tree Service LLC | 4419291 | 2 | 2 | IntrastateHazmat |
| Chase Moving And Service | 1737659 | 1 | 1 | Intrastate |
| Mario Trucking INC | 1900407 | 1 | 1 | Interstate |
| Dm Trucking | 2048318 | 1 | 1 | Interstate |
| Alex Always Express INC | 2117087 | 1 | 1 | Interstate |
| Material Transport Solutions | 2315012 | 1 | 1 | Intrastate |
| Gfour Productions LLC | 2333141 | 1 | 2 | Interstate |
| Henry's Old-Fashioned Kettle Korn | 2398172 | 1 | 1 | Interstate |
| Hidden Little Treasures Learning Center | 2477425 | 1 | 4 | Intrastate |
| Richard Verbano | 2492770 | 1 | 1 | Intrastate |
| Otto Pauls Transport & Service INC | 2563368 | 1 | 1 | Interstate |
| Prirmose School Of Julington Creek | 2943535 | 1 | 1 | Intrastate |
| Primrose School Of St Johns Forest | 2943539 | 1 | 1 | Intrastate |
| Dru Transport LLC | 2960275 | 1 | 1 | Interstate |
| Superdan Trucking LLC | 2962075 | 1 | 1 | Interstate |
| Aa 1 Mobile Service Plus LLC | 3017459 | 1 | 1 | Intrastate |
| Ddjd | 3075355 | 1 | 1 | Intrastate |
| Jg Enterprises LLC | 3076408 | 1 | 4 | Intrastate |
| D & T Transport Services LLC | 3200182 | 1 | 3 | IntrastateHazmat |
| Amr Transport II LLC | 3229389 | 1 | 1 | Interstate |
| Parrott Custom Hauling LLC | 3232164 | 1 | 1 | Intrastate |
| Kes Transportation Corp | 3277128 | 1 | 1 | Interstate |
| Ladybird Academy Of Saint Johns | 3289697 | 1 | 4 | Intrastate |
| Cml Exports And Moving Solutions INC | 3300481 | 1 | 2 | Intrastate |
| Mpl Fiber INC | 3457748 | 1 | 1 | Interstate |
| Services By Peppertree LLC | 3507985 | 1 | 1 | Intrastate |
| Dumpster Crushr Of Florida | 3526301 | 1 | 3 | Intrastate |
| Hache Express Transport LLC | 3663963 | 1 | 1 | Intrastate |
| Luggers Moving Of St Johns | 3678758 | 1 | 4 | Intrastate |
| Bjt Truck Express LLC | 3751149 | 1 | 1 | Intrastate |
| Mcintosh Estates INC | 3833496 | 1 | 1 | IntrastateHazmat |
| Go Minis Of Eastern Florida | 3856308 | 1 | 2 | Intrastate |
| Black Phoenix Transports LLC | 3892185 | 1 | 1 | Interstate |
| Durbin Creek Nursery | 3901749 | 1 | 1 | Intrastate |
| Around The Clock Transportation Services | 4062427 | 1 | 1 | Intrastate |
| Reef Auto Transport LLC | 4062552 | 1 | 1 | IntrastateHazmat |
| Jf3 General Services LLC | 4085706 | 1 | 1 | Intrastate |
| Master Moor Logistics LLC | 4142987 | 1 | 1 | Interstate |
| Seven Miles Transport INC | 4255721 | 1 | 1 | Interstate |
| Paradox Global LLC | 4265484 | 1 | 1 | Interstate |
| South Florida Towing LLC | 4266053 | 1 | 1 | IntrastateHazmat |
| Formula 7 Lds Transportation LLC | 4268298 | 1 | 1 | Interstate |
| Transmel LLC | 4273889 | 1 | 1 | Intrastate |
| Tokyo Transport LLC | 4276894 | 1 | 1 | Interstate |
| Kristof Land Management LLC | 4324335 | 1 | 1 | Intrastate |
| Zola Trucking LLC | 4330377 | 1 | 1 | Intrastate |
| J&l Trucking 365 LLC | 4336983 | 1 | 1 | Intrastate |
| Universal Family Transportation & Logistics INC | 4349443 | 1 | 2 | Intrastate |
| Tractor LLC | 4361310 | 1 | 1 | Interstate |
| Lakeview Contracting INC | 4385362 | 1 | 2 | Intrastate |
| PC Transportation Services LLC | 4385643 | 1 | 1 | Interstate |
| Better Future Aim LLC | 4396745 | 1 | 1 | Interstate |
| Jettts Transportation | 4404618 | 1 | 2 | Intrastate |
| Vilabel Transportation LLC | 4417555 | 1 | 1 | Interstate |
| Beam Hauling LLC | 4474858 | 1 | 1 | Intrastate |
| Rod's Seafood & Deli INC | 4480380 | 1 | 1 | Interstate |
| Chiva St Augustine | 4486811 | 1 | 6 | Intrastate |
| 2e2d LLC | 4493169 | 1 | 1 | Interstate |
| Arrow Logistics Group LLC | 4514269 | 1 | 1 | Interstate |
| Nova Haul Logistics LLC | 4558301 | 1 | 1 | Interstate |
| Bejarano Group LLC | 4559558 | 1 | 1 | Interstate |
| Spetter Hauling CO LLC | 4577916 | 1 | 1 | Intrastate |
| Nikolenko Transport Solution LLC | 5055379 | 1 | 1 | Interstate |
| Ascora Logistics LLC | 5198646 | 1 | 2 | Interstate |
| Karon LLC | 8233703 | 1 | 4 | Interstate |
| Oecs, LLC | 8721222 | 1 | 2 | Interstate |