| Publix | 439262 | 945 | 1,720 | InterstateHazmat |
| Southeast Independent Delivery Services INC | 842212 | 748 | 950 | Interstate |
| Saddle Creek Transportation INC | 1196553 | 409 | 535 | Interstate |
| Phoenix Logistics & Cold Storage LLC | 3348075 | 257 | 243 | Interstate |
| Viasys Utility Services INC | 818863 | 172 | 129 | Intrastate |
| Exxact Transport | 612401 | 91 | 96 | Interstate |
| Harrell's | 667489 | 73 | 87 | InterstateHazmat |
| Best Carrier Solutions INC | 2811363 | 72 | 70 | Interstate |
| Preferred Drilling Solutions, LLC | 6350736 | 71 | 244 | Interstate |
| Allied Crawford Steel INC | 432441 | 67 | 67 | Interstate |
| Springer Peterson Roofing & Sheet Metal INC | 1846304 | 62 | 69 | Intrastate |
| Cavalier Distributing | 2822279 | 39 | 38 | Intrastate |
| Fleetwing Distribution Services LLC | 1201193 | 33 | 18 | Intrastate |
| Midwestern Construction INC | 2269529 | 31 | 54 | Intrastate |
| Sandel Transport INC | 1937243 | 29 | 29 | Intrastate |
| Ameraparts International LLC | 813615 | 28 | 12 | Intrastate |
| Bender Transportation LLC | 4283876 | 28 | 38 | Interstate |
| Webbs Towing & Recovery Service | 300350 | 27 | 17 | InterstateHazmat |
| Amware Logistics Services Of Florida L L C | 747459 | 26 | 27 | Intrastate |
| Goddard Foodservice | 3724222 | 24 | 24 | Intrastate |
| Tech-Gas Solutions LLC | 3925618 | 24 | 19 | IntrastateHazmat |
| Sunrise Community Of Polk County INC | 4499228 | 24 | 65 | Interstate |
| Barstow Transportation LLC | 968462 | 22 | 20 | Interstate |
| Little Wheels INC | 804356 | 21 | 21 | Intrastate |
| Adena Lakeland LLC | 3556861 | 21 | 18 | Interstate |
| Enza Trucking LLC | 2956451 | 20 | 15 | Interstate |
| Battery USA INC | 861368 | 19 | 19 | Interstate |
| Engineered Spray Solutions LLC | 952038 | 19 | 24 | Intrastate |
| Mabe Production & Installation LLC | 1329646 | 19 | 56 | Intrastate |
| Larh Constructions LLC | 3894191 | 19 | 7 | Intrastate |
| F B M Transportation INC | 2496025 | 18 | 18 | Interstate |
| Bakers Transport Service Of Lakeland INC | 598111 | 17 | 19 | Intrastate |
| Dixie Signs INC | 845509 | 15 | 8 | Intrastate |
| Stronghold Plumbing & Septic | 3649877 | 15 | 2 | Intrastate |
| Florida Tile INC | 424879 | 14 | 11 | Interstate |
| Killebrew INC | 847663 | 14 | 9 | Intrastate |
| Averett Septic Tank CO INC | 1118368 | 14 | 17 | Intrastate |
| Two Men And A Truck | 1654865 | 14 | 10 | Interstate |
| Mid-State Industrial Maintenance | 883845 | 13 | 8 | Interstate |
| General Asphalt Paving LLC | 2260652 | 13 | 16 | Intrastate |
| Cargo Resources LLC | 2386140 | 13 | 13 | Interstate |
| Aeon Medical Transport LLC | 4224518 | 13 | 13 | Interstate |
| Quality Petroleum Corp | 899620 | 12 | 8 | IntrastateHazmat |
| Alwaysworken INC | 1094533 | 12 | 7 | Intrastate |
| Aj's Asphalt Svc LLC | 2582697 | 12 | 10 | Intrastate |
| Powerscreen Of Florida INC | 560188 | 11 | 9 | Interstate |
| The Ledger | 887441 | 11 | 12 | Intrastate |
| John Goddard Produce INC | 1139825 | 11 | 11 | Intrastate |
| San Juan Pools | 534134 | 10 | 10 | Interstate |
| Cement Products & Supply CO INC | 821751 | 10 | 8 | Intrastate |
| Crane & Rigging Of Florida | 864927 | 10 | 9 | Intrastate |
| Lakeland Crane Corporation | 884907 | 10 | 7 | Intrastate |
| Southern Softwoods INC | 992676 | 10 | 9 | Intrastate |
| D J Trusses Unlimited INC | 1046855 | 10 | 8 | Intrastate |
| Pouncey Sanitation And Recycling INC | 3020773 | 10 | 8 | Intrastate |
| Jacob's Express LLC | 3432668 | 10 | 4 | InterstateHazmat |
| M&b Logistic Services INC | 3637396 | 10 | 2 | Intrastate |
| Imperial Testing And Engineering INC | 3727961 | 10 | 24 | Intrastate |
| Yudith Trucking INC | 3918871 | 10 | 10 | Intrastate |
| Mile High Ltl LLC | 3996306 | 10 | 10 | Interstate |
| Three Knights Transport LLC | 4336113 | 10 | 5 | Interstate |
| Agrichargelogistics LLC | 4552094 | 10 | 0 | Interstate |
| C J Pallet CO INC | 292998 | 9 | 7 | Intrastate |
| Pallet Recycle | 573134 | 9 | 6 | Interstate |
| Lakeland Animal Nutrition INC | 823749 | 9 | 6 | Intrastate |
| Wood Mulch Products INC | 992671 | 9 | 7 | Intrastate |
| Lakeland Nutrition Group LLC | 1844861 | 9 | 11 | Intrastate |
| Joyce Wrecker Service LLC | 2163435 | 9 | 9 | Intrastate |
| Level Line Concrete, Inc. | 8623578 | 9 | 18 | Intrastate |
| Triple J Construction And Utilities LLC | 9343329 | 9 | 4 | Intrastate |
| Advanced Aluminum Of Polk County INC | 465100 | 8 | 4 | Interstate |
| Interstate Battery System Of Lakeland INC | 837597 | 8 | 9 | Intrastate |
| A R C Towing Service LLC | 1810961 | 8 | 8 | Intrastate |
| Joyce Automotive | 3405141 | 8 | 10 | Intrastate |
| Acs 2 LLC | 3757702 | 8 | 6 | Interstate |
| Interstate Battery Systems Of Lakeland | 4450426 | 8 | 6 | IntrastateHazmat |
| Southern Milling & Lumber INC | 512910 | 7 | 5 | Intrastate |
| Mid-State Truss Company INC | 856830 | 7 | 8 | Intrastate |
| D S Palmer Trucking | 1189754 | 7 | 6 | Intrastate |
| Cargo Solutions Of Florida | 1247115 | 7 | 7 | Intrastate |
| Custom Linen Solutions | 1275001 | 7 | 9 | Intrastate |
| Shane Tractor Service INC | 1337858 | 7 | 7 | Intrastate |
| Edely Transportation LLC | 1814252 | 7 | 7 | Intrastate |
| Potable On Demand Storage Pods | 2024573 | 7 | 10 | Intrastate |
| Jesse Cole Trucking INC | 2575572 | 7 | 7 | Intrastate |
| Mahoney's Transport USA LLC | 2833710 | 7 | 3 | Intrastate |
| Escobar Logistics LLC | 3064094 | 7 | 7 | Intrastate |
| Green Well Drilling LLC | 3216848 | 7 | 4 | Intrastate |
| One More Child | 3343794 | 7 | 20 | Intrastate |
| Roggen Clyne Development INC | 3993702 | 7 | 7 | Intrastate |
| Carol Concrete LLC | 4061186 | 7 | 7 | Interstate |
| Wattworks Energy LLC | 4074280 | 7 | 4 | Intrastate |
| Dm Auto Movers INC | 4223214 | 7 | 7 | InterstateHazmat |
| S&w Towing & Recovery Services LLC | 4248244 | 7 | 16 | Intrastate |
| Mission Towing And Recovery LLC | 4463194 | 7 | 6 | Intrastate |
| Buddy's Home Furnishings | 6208589 | 7 | 8 | Intrastate |
| Batteries By Fisher INC | 560265 | 6 | 11 | IntrastateHazmat |
| R Cline INC | 861371 | 6 | 7 | Intrastate |
| Browning Moving & Storage | 882348 | 6 | 7 | Intrastate |
| Ftd Construction INC | 1118326 | 6 | 9 | Intrastate |