| 1 Stop Enterprises INC | 1714438 | 1,200 | 1,200 | Intrastate |
| Fleetgenius LLC | 3490693 | 110 | 110 | Interstate |
| Crawford Landscaping Group LLC | 1811825 | 90 | 55 | Intrastate |
| Naples Transportation & Tours | 1393792 | 84 | 75 | Interstate |
| 4 Star Trucking Company LLC | 2349941 | 70 | 70 | Intrastate |
| Ibc | 2556568 | 66 | 66 | Interstate |
| Greenscapes Of Southwest Florida INC | 2530934 | 65 | 100 | Intrastate |
| R T R Trucking | 1054255 | 52 | 52 | Intrastate |
| Oakes Farms Food Service & Distribution | 3390758 | 51 | 50 | Intrastate |
| Better Roads INC | 820397 | 41 | 36 | IntrastateHazmat |
| Umi The Source | 1232995 | 41 | 24 | Interstate |
| Commercial Concrete Systems LLC | 1355026 | 41 | 56 | InterstateHazmat |
| Northern Litho INC | 1058880 | 39 | 39 | Interstate |
| Oakesfarms Food & Distribution Services LLC | 3830083 | 37 | 38 | Intrastate |
| Kctransportationllc | 2340206 | 36 | 26 | Intrastate |
| Askar Energy | 2838074 | 34 | 24 | IntrastateHazmat |
| Quality Enterprises USA INC | 1023103 | 33 | 32 | InterstateHazmat |
| Coastal Beverage Ltd | 804909 | 31 | 68 | Intrastate |
| Best Electric Air Conditioning & Plumbing LLC | 4348281 | 31 | 31 | Intrastate |
| Na | 3916046 | 30 | 30 | Interstate |
| Bonness Inc. | 5824350 | 29 | 5 | Intrastate |
| Qe Concrete | 3919085 | 27 | 36 | Intrastate |
| Paramount Asphalt Sealcoating Corporation | 3509904 | 26 | 10 | Intrastate |
| Dixie Trucking INC | 1054981 | 25 | 25 | Intrastate |
| Jw Craft Portable Restrooms | 1159557 | 25 | 21 | Intrastate |
| Dixie Dirt Movers INC | 1259696 | 25 | 25 | Intrastate |
| Dmva Trucking LLC | 3574443 | 25 | 25 | Interstate |
| Crown Logistics INC | 1063121 | 24 | 25 | Interstate |
| Commercial Clean Up Enterprises INC | 814663 | 23 | 15 | Intrastate |
| Environmental Turnkey Solutions LLC | 2345244 | 23 | 24 | Intrastate |
| Overland Transport INC | 1543314 | 20 | 20 | Intrastate |
| Omni Construction LLC | 3592253 | 20 | 13 | Interstate |
| American Farms LLC | 696619 | 19 | 19 | Intrastate |
| Shoreline Flooring Supplies | 1204994 | 19 | 19 | Intrastate |
| A R Landscaping Services INC | 1366142 | 19 | 19 | Intrastate |
| Naples Lumber & Supply Company INC | 861526 | 18 | 11 | Intrastate |
| United States Transport Towing & Recovery LLC | 3168156 | 18 | 14 | IntrastateHazmat |
| Evans Oil Company LLC | 822404 | 17 | 36 | IntrastateHazmat |
| Incredible Fresh Cpt Jerrys Intl | 1151862 | 16 | 17 | Intrastate |
| Bentley Electric Company Of Naples Fl INC | 1623030 | 16 | 15 | Intrastate |
| Cisco | 2963103 | 16 | 14 | Intrastate |
| Sunshine Excavators INC | 814205 | 15 | 15 | Intrastate |
| J & C Drainfield LLC | 1139989 | 15 | 14 | Intrastate |
| Swfl Delivery Services LLC | 3454487 | 15 | 15 | Intrastate |
| Naples Disposal LLC | 3546260 | 15 | 14 | Intrastate |
| Wts Transport | 4555059 | 15 | 15 | Interstate |
| Allied Crane Service INC | 830571 | 14 | 15 | Intrastate |
| Mbi Tours | 247660 | 13 | 16 | Interstate |
| Lainhart Excavating INC | 804382 | 13 | 10 | Intrastate |
| O'green Concrete Pumping LLC | 873332 | 13 | 13 | Interstate |
| Bonness INC | 1350884 | 13 | 8 | IntrastateHazmat |
| Busk & Associates INC | 1139809 | 12 | 14 | Intrastate |
| Anytown Tree Service | 1678398 | 12 | 12 | Intrastate |
| Combs Oil Company | 589370 | 11 | 6 | IntrastateHazmat |
| Topiary Creations INC | 1038631 | 11 | 10 | Interstate |
| Peluso Movers INC | 1941779 | 11 | 7 | Intrastate |
| Naples Disposal | 2953794 | 11 | 6 | Intrastate |
| Emerald Landscaping Company Of Sw Fl INC | 3164949 | 11 | 7 | Intrastate |
| Moon Valley Nursery Of Florida LLC | 4158163 | 11 | 6 | Intrastate |
| Soave Hospitality Group INC | 4235312 | 11 | 19 | Intrastate |
| Tri County Contracting | 1070866 | 10 | 10 | Intrastate |
| Bm Gray INC | 1094282 | 10 | 7 | Intrastate |
| Ddg | 1424178 | 10 | 5 | Intrastate |
| Kam Concrete Pumping INC | 1499108 | 10 | 18 | Intrastate |
| Culvert Works INC | 1539439 | 10 | 9 | Intrastate |
| Crush Aggregates Transportation INC | 1843891 | 10 | 10 | Intrastate |
| Bald Eagle Towing | 2196348 | 10 | 7 | Intrastate |
| Castilla Roofing INC | 2783048 | 10 | 15 | Intrastate |
| First Fleet Express Services LLC | 3534203 | 10 | 5 | Interstate |
| Hope CO Investment LLC | 3799662 | 10 | 2 | Intrastate |
| Statewide Xpress LLC | 3903302 | 10 | 12 | Interstate |
| Lang Logistics INC | 4195177 | 10 | 7 | Interstate |
| Osprey-Gulf Shore Building Materials INC | 880036 | 9 | 9 | Intrastate |
| Allen Concrete & Masonry INC | 924124 | 9 | 10 | Intrastate |
| The Equipment Source | 966802 | 9 | 7 | Intrastate |
| Reliable Disposal | 1432541 | 9 | 9 | Intrastate |
| Seminole Petroleum | 2406865 | 9 | 18 | IntrastateHazmat |
| Aero Trucking Group Corp | 2464812 | 9 | 8 | Intrastate |
| Golden Army Logistics LLC | 3980727 | 9 | 6 | Interstate |
| Warehouse Services INC | 615178 | 8 | 8 | Intrastate |
| Wm C Huff Moving & Storage | 714491 | 8 | 8 | Interstate |
| Earthworks Of Naples INC | 824363 | 8 | 8 | Intrastate |
| Hershell's Crane Service INC | 886795 | 8 | 6 | Intrastate |
| Pena Trucking & Excavating | 929900 | 8 | 6 | Intrastate |
| Reinery Trucking INC | 1061562 | 8 | 8 | Intrastate |
| Golf Turf Applications INC | 1213946 | 8 | 4 | Interstate |
| Tropic Disposal Services INC | 1351614 | 8 | 7 | Intrastate |
| Aaron Goddard Construction INC | 1610540 | 8 | 8 | Intrastate |
| Incrediblefresh Seafood | 1624293 | 8 | 4 | Intrastate |
| Martin's Loading LLC | 1962313 | 8 | 6 | Intrastate |
| Gulf Coast Roofing CO INC | 1976789 | 8 | 8 | Intrastate |
| Acosta Harvesting | 2259847 | 8 | 1 | Intrastate |
| Gulf Coast Deli Provisions INC | 2317102 | 8 | 10 | Intrastate |
| Angel Express Cargo Corp | 2979273 | 8 | 8 | Intrastate |
| Delgado & Sons Enterprise LLC | 2981773 | 8 | 8 | Intrastate |
| American Five Star Transport LLC | 3325559 | 8 | 8 | Interstate |
| Discount Dumpster Services INC | 4500673 | 8 | 4 | Intrastate |
| Boys & Girls Club Ofcollier County Florida Inc. | 6110240 | 8 | 40 | Intrastate |
| B J Excavating Enterprises INC | 820671 | 7 | 5 | IntrastateHazmat |
| Collier Recycling Services | 823775 | 7 | 6 | Intrastate |