| A & A Truck Brokers INC | 1151771 | 177 | 177 | Intrastate |
| United Mechanical LLC | 3526492 | 106 | 72 | Intrastate |
| Suncoast Beverage Sales LLC | 1020069 | 99 | 98 | Intrastate |
| Schwab Ready-Mix INC | 506234 | 96 | 64 | Intrastate |
| Drake Ready Mix INC | 1433166 | 87 | 66 | Intrastate |
| Collier Paving & Concrete | 2513484 | 68 | 54 | Intrastate |
| Southwest Waste Services LLC | 3560621 | 68 | 68 | Intrastate |
| Lmsg Logistics Incorporated | 3206097 | 60 | 90 | Interstate |
| Power Support Holdings INC | 3998800 | 60 | 73 | Intrastate |
| Cypress Trucking INC | 804927 | 58 | 57 | Intrastate |
| Southwest Concrete Corp | 3854325 | 52 | 60 | Intrastate |
| Sei Logistics LLC | 3307013 | 50 | 71 | InterstateHazmat |
| Backbay Construction INC | 2526142 | 49 | 10 | Intrastate |
| Castlegate Building Corporation | 3225952 | 49 | 16 | Intrastate |
| Sunny Grove Landscaping And Nursery INC | 1152033 | 48 | 216 | Intrastate |
| Sunshine Recycling Services Of Southwest Florida LLC | 1011675 | 40 | 37 | Intrastate |
| Ckc Delivery Service LLC | 3195674 | 40 | 85 | Interstate |
| Campbell Roofing & Sheet Metal INC | 1730422 | 39 | 80 | Intrastate |
| Extreme Demolition And Land Clearing LLC | 3141193 | 38 | 26 | Intrastate |
| S W Trucking INC | 808069 | 36 | 25 | Intrastate |
| American Water Services Residual Management | 975498 | 35 | 38 | Intrastate |
| Fas Transport LLC | 4056211 | 35 | 75 | Interstate |
| Juniper Landscaping Of Bradenton | 2052042 | 34 | 34 | Intrastate |
| Cougar Cutting INC | 2579976 | 34 | 48 | Intrastate |
| Cougar Contracting LLC | 3297910 | 28 | 23 | Intrastate |
| Artesian Pool Construction INC | 1956026 | 26 | 32 | IntrastateHazmat |
| Edison Oil Company | 835205 | 25 | 24 | IntrastateHazmat |
| American Infrastructure Services INC | 3123010 | 23 | 18 | Intrastate |
| Tomahawk Construction LLC | 1332464 | 22 | 10 | IntrastateHazmat |
| Gary Honas Trucking LLC | 1692965 | 22 | 20 | Intrastate |
| Gatewood Glass INC | 3264543 | 21 | 35 | Intrastate |
| Improve Soil Distributor | 1181124 | 20 | 20 | Intrastate |
| Eagle Ready Mix LLC | 1544076 | 20 | 20 | Intrastate |
| Two Men And A Truck | 2951563 | 20 | 35 | Interstate |
| Premier Portables | 3953861 | 20 | 20 | Intrastate |
| Crowther Roofing & Sheet Metal | 824831 | 19 | 21 | IntrastateHazmat |
| Sweeping Company Of Florida LLC | 1569587 | 19 | 20 | Intrastate |
| Stag Leasing INC | 874633 | 18 | 18 | IntrastateHazmat |
| Forestry Resources Transportation Solutions LLC | 1101112 | 18 | 18 | Intrastate |
| Cl Noonan Disposal INC | 1529699 | 18 | 17 | Intrastate |
| Harry Chapin Food Bank Of Southwest Florida INC | 2968741 | 18 | 16 | Intrastate |
| Waste Management Training Center | 364781 | 17 | 13 | InterstateHazmat |
| County Waste LLC | 964447 | 17 | 10 | Interstate |
| Florida Ready-Mix Concrete LLC | 1336209 | 17 | 14 | Intrastate |
| Honest And True Moving Crew LLC | 2146168 | 17 | 14 | Intrastate |
| Shell Point Retirement Community | 3906085 | 17 | 17 | Interstate |
| Fort Myers Trucking INC | 725730 | 16 | 18 | Interstate |
| Youngquist Brothers LLC | 956257 | 16 | 19 | Intrastate |
| Pavement Maintenance LLC | 2354323 | 16 | 50 | Intrastate |
| Hannula Landscaping & Irrigation Services | 7056974 | 16 | 4 | Intrastate |
| Capstone Hme Of Florida | 1254242 | 15 | 15 | Intrastate |
| Christian Playmates Preschool And Daycare INC | 2557621 | 15 | 3 | Intrastate |
| Alligator Towing And Transport LLC | 4254785 | 15 | 15 | IntrastateHazmat |
| Oremus Material Fl | 4364011 | 15 | 15 | Intrastate |
| Wells And Water Systems INC | 1172179 | 14 | 15 | Intrastate |
| Kuhlman Concrete LLC | 1432016 | 14 | 11 | Intrastate |
| Shore To Shore Freightlines INC | 1528382 | 14 | 3 | Intrastate |
| Lee Health System INC | 4430335 | 14 | 50 | IntrastateHazmat |
| Titan Concrete & Utilities INC | 4111741 | 13 | 2 | Intrastate |
| Stevens & Layton INC | 402320 | 12 | 2 | IntrastateHazmat |
| Hurricane Transport | 939174 | 12 | 12 | Intrastate |
| Tincher Concrete Construction INC | 1155622 | 12 | 12 | Intrastate |
| Hannula Landscaping & Irrigation INC | 1763248 | 12 | 18 | Intrastate |
| USA Waste Services LLC | 2285298 | 12 | 7 | Intrastate |
| Road Trucks Solutions Corp | 2468970 | 12 | 12 | Intrastate |
| Happy Foods Of Sw Fla INC | 2534196 | 12 | 11 | Intrastate |
| Nje Concrete Pumping LLC | 3957640 | 12 | 7 | Intrastate |
| Leesar INC | 1324394 | 11 | 23 | Intrastate |
| Russ Berner Construction INC | 1334449 | 11 | 13 | Intrastate |
| Null | 2576292 | 11 | 3 | Intrastate |
| Bob Dean Supply INC | 814171 | 10 | 6 | Intrastate |
| Ehcinc | 833623 | 10 | 8 | IntrastateHazmat |
| Gulf Paving CO INC | 1159218 | 10 | 9 | Intrastate |
| Academy Transport LLC | 2060465 | 10 | 5 | Intrastate |
| Gulfshore Trucking LLC | 2420817 | 10 | 10 | Intrastate |
| I2 Visual | 3021806 | 10 | 10 | Interstate |
| Pmd Trucking Services LLC | 3189859 | 10 | 10 | Interstate |
| Perfection Lawn And Pest Control INC | 4402129 | 10 | 13 | Intrastate |
| Village Land Clearing & Fill INC | 886307 | 9 | 5 | Intrastate |
| Green Care Recycling | 2069902 | 9 | 4 | Intrastate |
| Robb & Stucky International | 2208613 | 9 | 10 | Intrastate |
| Jp Harvesting Div II LLC | 3829458 | 9 | 7 | Intrastate |
| Titan Contracting LLC | 4240611 | 9 | 10 | Intrastate |
| Kennedy International Logistics Collier | 4259852 | 9 | 9 | Intrastate |
| Landscape Logistics LLC | 4398238 | 9 | 9 | Interstate |
| Metro Ice Company INC | 872907 | 8 | 8 | Intrastate |
| Ad-Ler Roofing Company | 1325388 | 8 | 8 | Intrastate |
| Walrok INC | 1476459 | 8 | 6 | Intrastate |
| Stymie Holdings LLC | 1991316 | 8 | 1 | Intrastate |
| Moboy Leasing LLC | 2084182 | 8 | 8 | Intrastate |
| Scotlynn Transport LLC | 2386725 | 8 | 10 | Interstate |
| Accurate Grading INC | 2485101 | 8 | 8 | Intrastate |
| Greenco Vegetation Recycling | 2969002 | 8 | 9 | Intrastate |
| Shoreline Lumber INC | 3040678 | 8 | 10 | Intrastate |
| Jesus Maria 25 Delivery Corp | 3112982 | 8 | 8 | Intrastate |
| Southwest Structural INC | 3447711 | 8 | 8 | Intrastate |
| Maj Contracting INC | 955894 | 7 | 4 | Intrastate |
| Labelle Well Drilling & Water Sysytems INC | 1541991 | 7 | 11 | Intrastate |
| Johnson Engineering INC | 1744523 | 7 | 11 | Intrastate |
| Two Men And A Truck | 2163582 | 7 | 10 | Intrastate |