| Anderson Columbia CO INC | 545147 | 356 | 338 | IntrastateHazmat |
| Cdt USA INC | 1614853 | 218 | 109 | Interstate |
| Rdh Trucking INC | 1106515 | 46 | 84 | Interstate |
| Busy Bee Bulk Fuels LLC | 2906531 | 31 | 19 | InterstateHazmat |
| Dct INC | 672423 | 25 | 21 | Interstate |
| Cushman's Custom Services INC | 1264009 | 25 | 25 | Intrastate |
| A Truckline | 3320376 | 23 | 16 | Interstate |
| Columbia Ready Mix | 3038370 | 22 | 20 | Intrastate |
| City Of Lake City | 221600 | 18 | 10 | Intrastate |
| Ca Boone Construction INC | 1119985 | 11 | 11 | Intrastate |
| Fts Limousine | 2435799 | 11 | 12 | Intrastate |
| Columbia Salvage INC | 833058 | 10 | 10 | Interstate |
| Utility Trucks & Equipment INC | 2871642 | 10 | 10 | IntrastateHazmat |
| Curt's Construction INC | 845363 | 9 | 9 | Interstate |
| Jims Auto Service | 1160089 | 9 | 8 | Interstate |
| Advanced Project Solutions LLC | 2265282 | 9 | 5 | Interstate |
| Lakeside Transportation INC | 2298248 | 9 | 6 | Interstate |
| Southwest Georgia Oil Company | 2638916 | 9 | 9 | Interstate |
| Hunter Oil | 339768 | 8 | 4 | IntrastateHazmat |
| Bryants Tires And Towingbryants Towing | 657604 | 8 | 8 | IntrastateHazmat |
| Bubba's Site Prep | 935750 | 8 | 6 | Intrastate |
| Hughes Well Drilling & Pump Service LLC | 2159002 | 8 | 3 | Intrastate |
| Southeast Milk INC | 151614 | 7 | 13 | InterstateHazmat |
| Lake City Industries INC | 231625 | 7 | 6 | Intrastate |
| Premium Truck Services LLC | 4015031 | 7 | 7 | Interstate |
| Mccray's Construction | 912000 | 6 | 4 | Intrastate |
| Green Dynamix | 1047375 | 6 | 5 | Interstate |
| Mow Hog Mowing And Grading LLC | 1054365 | 6 | 2 | IntrastateHazmat |
| North Florida Septic Tankinc | 1199220 | 6 | 10 | IntrastateHazmat |
| B & C Sales INC | 1744481 | 6 | 10 | Intrastate |
| Solid Foundations LLC | 3732084 | 6 | 11 | Interstate |
| As Equipment & Rental LLC | 3934207 | 6 | 16 | Interstate |
| Orange State Towing & Recovery | 3996163 | 6 | 1 | IntrastateHazmat |
| Hei Generators | 4315416 | 6 | 24 | Intrastate |
| Remco National LLC | 4574558 | 6 | 6 | Interstate |
| Dicks Realty | 974396 | 5 | 3 | Intrastate |
| Davis Towing & Recovery | 1215832 | 5 | 3 | IntrastateHazmat |
| Sapp Salvage LLC | 2030700 | 5 | 4 | Intrastate |
| Feagle Logging LLC | 2181080 | 5 | 5 | Interstate |
| Bbk Contractors INC | 2899948 | 5 | 5 | Intrastate |
| B&b Contractors Of Florida INC | 3171358 | 5 | 5 | Intrastate |
| 238 West INC | 3372432 | 5 | 3 | Intrastate |
| Evermark Transport, Llc. | 8914172 | 5 | 5 | InterstateHazmat |
| Wilson's Containers | 258030 | 4 | 4 | Intrastate |
| Tnt Transport | 766474 | 4 | 3 | Interstate |
| A A A Construction Company | 818336 | 4 | 4 | Intrastate |
| Thomas Trucking | 826555 | 4 | 4 | Intrastate |
| Summerlin Roofing INC | 1141215 | 4 | 1 | Intrastate |
| George Moseley Spreading Service INC | 1185024 | 4 | 4 | Intrastate |
| Southern Aggregate LLC | 1274557 | 4 | 4 | Intrastate |
| Peloni's Pumping & Portable Toilets INC | 1760397 | 4 | 3 | Intrastate |
| Williams Wildland Fire Services LLC | 2383438 | 4 | 4 | Intrastate |
| Suwannee Valley 4cs | 2627027 | 4 | 7 | Intrastate |
| Green Acres Learning Center | 2785902 | 4 | 5 | Intrastate |
| Tripple G Land Specialists LLC | 3186183 | 4 | 1 | Intrastate |
| Cab Contracting LLC | 3305008 | 4 | 1 | Intrastate |
| Rucker's Tree Services LLC | 3509956 | 4 | 2 | Intrastate |
| Columbia Containers & Storage LLC | 3661438 | 4 | 2 | Interstate |
| Hotshot Driving Services LLC | 3766455 | 4 | 4 | InterstateHazmat |
| K & J Livestock Hauling INC | 3864655 | 4 | 4 | Interstate |
| M&m Trucking INC | 552639 | 3 | 3 | Interstate |
| Florida Fill & Grading INC | 813709 | 3 | 4 | Intrastate |
| Adams LP Gas Of Lake City | 815276 | 3 | 2 | Intrastate |
| Greg Willems Trucking & Landworks | 844750 | 3 | 1 | Intrastate |
| Meeks Grain & Milling INC | 858092 | 3 | 2 | Intrastate |
| D L T Trucking | 863411 | 3 | 1 | Intrastate |
| L & R Distributors INC | 908115 | 3 | 4 | Intrastate |
| Dales Excavation INC | 1185059 | 3 | 3 | Intrastate |
| Nettles Sausage INC | 1260628 | 3 | 1 | Interstate |
| Sattler's Concessions | 1377008 | 3 | 1 | Interstate |
| Lake City Management Group INC | 1417766 | 3 | 2 | Intrastate |
| Southeastern Paving And Construction LLC | 1501603 | 3 | 2 | Intrastate |
| Evachek's Tree Sources INC | 1567004 | 3 | 2 | Intrastate |
| Fords Septic Tank Service LLC | 1648692 | 3 | 6 | Intrastate |
| Lewis Truckin INC | 2407051 | 3 | 1 | Intrastate |
| E & S Equipment Sales & Surveying | 2414571 | 3 | 2 | Interstate |
| Castle Hill Academy LLC | 2498616 | 3 | 7 | Intrastate |
| Circle C Corp Ag Ranch Timber INC | 3197128 | 3 | 1 | Intrastate |
| American Towing & Wrecker Services INC | 3225912 | 3 | 3 | IntrastateHazmat |
| Mowing- Tractor Services And Auto Transport | 3421133 | 3 | 2 | Intrastate |
| Thomas Trucking And Transport LLC | 3427366 | 3 | 2 | Intrastate |
| Wj Transport | 3548670 | 3 | 3 | Interstate |
| Aj Boss Logistics INC | 3591085 | 3 | 3 | IntrastateHazmat |
| Davis Site Prep | 3750153 | 3 | 1 | Intrastate |
| Team Green Towing And Recovery | 4046942 | 3 | 2 | Intrastate |
| Jay Five Express LLC | 9085652 | 3 | 4 | Interstate |
| Joses Metal Works Llc. | 9394944 | 3 | 12 | Interstate |
| North Florida Welding Supply INC | 509838 | 2 | 1 | IntrastateHazmat |
| Case Truck Lines | 578150 | 2 | 2 | Interstate |
| Boyette Grain Feed & Seed LLC | 817056 | 2 | 1 | Intrastate |
| Signcraft & More INC | 877812 | 2 | 1 | Intrastate |
| Suncoast Transport Enterprises INC | 885761 | 2 | 2 | Interstate |
| Florida's Finest Mobile Home Setup | 887533 | 2 | 1 | Intrastate |
| Brian's Bulldozing | 967725 | 2 | 1 | Intrastate |
| Interlochen LLC | 996655 | 2 | 2 | Interstate |
| Columbia Mobile Home Service | 1003620 | 2 | 2 | Intrastate |
| Privette Enterprises INC | 1026188 | 2 | 2 | Intrastate |
| The Moss Man | 1074642 | 2 | 1 | Intrastate |
| Dredgeco Mine Services INC | 1117994 | 2 | 2 | Intrastate |
| Graham & Sons Electric INC | 1118439 | 2 | 3 | Intrastate |