| Gfl Environmental | 1793162 | 143 | 132 | Intrastate |
| 02b Kids | 3229520 | 102 | 102 | Intrastate |
| Gaston | 1037784 | 51 | 57 | InterstateHazmat |
| Oasis Landscape Services INC | 2902653 | 51 | 51 | IntrastateHazmat |
| A Candies Coachworks INC | 1515652 | 36 | 50 | Interstate |
| Hospital | 3060466 | 19 | 19 | Intrastate |
| Wg Johnson And Son INC | 463934 | 16 | 15 | IntrastateHazmat |
| Jennymoon Construction LLC | 4467398 | 16 | 15 | Intrastate |
| Orlando Millan Transportation LLC | 9617076 | 14 | 6 | Intrastate |
| St John's Beverage CO INC | 405559 | 13 | 1 | Intrastate |
| Florence Recycling | 1258960 | 13 | 13 | Intrastate |
| Ruth Jean Enterprises INC | 3633223 | 13 | 7 | IntrastateHazmat |
| Andrews Construction | 817388 | 12 | 9 | Intrastate |
| Lewis Oil Company INC | 856609 | 12 | 16 | IntrastateHazmat |
| Almond Oil Company | 348788 | 11 | 10 | InterstateHazmat |
| Elite Towing | 1152960 | 11 | 16 | InterstateHazmat |
| Gainesville Ice Service INC | 814118 | 10 | 6 | Intrastate |
| Watson Towing | 1395170 | 10 | 10 | Intrastate |
| B & M Equipment Rental & Sales | 3627189 | 10 | 6 | Interstate |
| Usi | 868642 | 9 | 17 | Intrastate |
| Shelby's Towing | 885954 | 9 | 3 | IntrastateHazmat |
| Painter Crane & Rigging | 900843 | 9 | 5 | Intrastate |
| Crom LLC | 158607 | 8 | 8 | InterstateHazmat |
| A Turner Moving & Storage INC | 813497 | 8 | 6 | Intrastate |
| Scherer Construction Of North Florida LLC | 878430 | 8 | 7 | IntrastateHazmat |
| Max's Towing LLP | 1331579 | 8 | 5 | Intrastate |
| Painter Crane And Rigging CO | 1594519 | 8 | 8 | Intrastate |
| Grays Tree Service | 1814078 | 8 | 2 | Intrastate |
| Gasp | 3164465 | 8 | 6 | Intrastate |
| Theissen Training Systems | 3954289 | 8 | 10 | Interstate |
| Wilson Exterior Cleaning | 4031190 | 8 | 2 | Intrastate |
| Ridgway Roof Truss CO | 452081 | 7 | 4 | Interstate |
| Myers Brothers Septic Myers Well Drilling | 834946 | 7 | 6 | Intrastate |
| Perma Fix Of Florida INC | 1005731 | 7 | 6 | InterstateHazmat |
| Abc Tree & Stump Removal INC | 1211479 | 7 | 5 | Intrastate |
| Gmg Transport | 1419949 | 7 | 6 | Intrastate |
| Ct Mechanical | 3936020 | 7 | 9 | Intrastate |
| Central Florida Contractor Services INC | 869337 | 6 | 5 | Intrastate |
| Perry Roofing INC | 873471 | 6 | 14 | Intrastate |
| Contractors Supply | 1212327 | 6 | 6 | Intrastate |
| Two Men And A Truck | 1669783 | 6 | 12 | InterstateHazmat |
| Community Waste Services INC | 3006194 | 6 | 5 | IntrastateHazmat |
| Letzz Ride LLC | 4059526 | 6 | 5 | Intrastate |
| Bunt Backline Event Services LLC | 4109073 | 6 | 15 | Interstate |
| Mad Max Driveaway Trucking | 4429177 | 6 | 8 | Intrastate |
| Moffos Towing & Recovery | 4522454 | 6 | 5 | Intrastate |
| Northwood Towing & Repair | 805869 | 5 | 4 | Intrastate |
| Gainesville Neon & Signs INC | 827617 | 5 | 5 | Intrastate |
| Hnsr Towing & Transport INC | 2077687 | 5 | 6 | Intrastate |
| Workman Forestry LLC | 3261036 | 5 | 4 | Intrastate |
| 2 College Brothers Of Gainesville | 4063852 | 5 | 4 | Interstate |
| Gatorback Towing And Recovery LLC | 4114378 | 5 | 6 | IntrastateHazmat |
| Dampier Septic Tank CO INC | 818883 | 4 | 2 | Intrastate |
| Gator Building Materials INC | 844515 | 4 | 5 | Intrastate |
| Tropical Recycling INC | 876385 | 4 | 2 | Intrastate |
| Taylor's Moving & Storage | 988084 | 4 | 4 | Intrastate |
| Central Florida Towing INC | 996791 | 4 | 4 | Intrastate |
| Ecopest Termite & Pest Control INC | 1277789 | 4 | 4 | Intrastate |
| Akira Wood INC | 2149442 | 4 | 2 | Intrastate |
| Koogler And Associates INC | 2381551 | 4 | 6 | Interstate |
| The Gainesville Sun | 2555565 | 4 | 4 | Intrastate |
| Ward Timber Trucking LLC | 2636963 | 4 | 4 | InterstateHazmat |
| Hamilton Custom Services LLC | 2836342 | 4 | 6 | Interstate |
| Afm Trucking LLC | 3096805 | 4 | 5 | Interstate |
| Top Shelf Interior Solutions | 3347266 | 4 | 4 | Interstate |
| Moffo's Towing And Recovery | 3490095 | 4 | 3 | IntrastateHazmat |
| Ox Strong Moving | 3588230 | 4 | 11 | Interstate |
| Lrp Trucking LLC | 3756617 | 4 | 4 | Intrastate |
| Climber Steve's Tree Care LLC | 3888743 | 4 | 3 | Intrastate |
| Decove Enterprises LLC | 3985577 | 4 | 4 | Interstate |
| Vo's Consulting LLC | 4295894 | 4 | 4 | Intrastate |
| Hogtown Underground INC | 832088 | 3 | 3 | Intrastate |
| Gainesville Paving INC | 833184 | 3 | 3 | Intrastate |
| University Ice | 834530 | 3 | 2 | Intrastate |
| Gainesville Pest Control INC | 884522 | 3 | 4 | Intrastate |
| Underwater Services International | 925808 | 3 | 3 | Interstate |
| Central Crane & Rigging INC | 959873 | 3 | 6 | Intrastate |
| Gaston Tree Service LLC | 1037786 | 3 | 3 | Interstate |
| Hills Concrete Pumping LLC | 1107990 | 3 | 3 | Intrastate |
| Ray's Metal Works INC | 1151184 | 3 | 2 | Interstate |
| Interstate Battery System Of North Central Fl INC | 1411414 | 3 | 3 | IntrastateHazmat |
| A Candies Limousine | 1428059 | 3 | 3 | Intrastate |
| Central Florida Drywall & Plastering INC | 1486453 | 3 | 6 | Intrastate |
| Dowling Signs | 1695651 | 3 | 2 | Intrastate |
| 24 Trucking | 1788284 | 3 | 3 | Interstate |
| Advanced Recovery Specialist | 2094794 | 3 | 1 | Intrastate |
| A 1 Eagle Hauling INC | 2352824 | 3 | 1 | Intrastate |
| Maaad Trucking Services INC | 3229573 | 3 | 3 | Interstate |
| Dusty Roads Trucking INC | 3299361 | 3 | 3 | Intrastate |
| Spartan Towing INC | 3306471 | 3 | 4 | IntrastateHazmat |
| Mnm Freight LLC | 3413412 | 3 | 3 | Interstate |
| Hall & Sons Trucking LLC | 3478332 | 3 | 2 | Intrastate |
| Swill Motor Freights INC | 3545045 | 3 | 1 | Interstate |
| Gator Tree Service INC | 3545246 | 3 | 3 | Intrastate |
| College Hunks Hauling Junk And Moving | 3763522 | 3 | 1 | Intrastate |
| Aps Logistics LLC | 3768303 | 3 | 2 | Intrastate |
| Chekk LLC | 3784550 | 3 | 6 | Intrastate |
| Thompson's Sod INC | 3924013 | 3 | 3 | Interstate |
| Above And Beyond Logistics LLC | 4213039 | 3 | 2 | Intrastate |
| Emirold Logistics LLC | 4310408 | 3 | 1 | Interstate |