| Adams Construction Company | 339391 | 308 | 200 | Interstate |
| Lawrence | 84337 | 297 | 318 | Interstate |
| Richardson-Wayland-Ferreira LLC | 4176579 | 200 | 185 | InterstateHazmat |
| Fleetmaster Express Incorporated | 404732 | 150 | 151 | Interstate |
| Lanford Brothers Company INC | 6779 | 129 | 90 | InterstateHazmat |
| Grand Home Furnishings | 608860 | 110 | 92 | Interstate |
| Branch Civil INC | 32509 | 90 | 87 | InterstateHazmat |
| Gerdau | 15802 | 89 | 65 | InterstateHazmat |
| Associated Asphalt Transport LLC | 235687 | 64 | 56 | Interstate |
| Abbott Bus Lines INC | 84283 | 50 | 61 | Interstate |
| Boxley Readymix LLC | 3750775 | 41 | 30 | Interstate |
| Carilion Clinic | 385797 | 40 | 11 | Interstate |
| Tebarge Seed Walker Supply & Corbin Turf Ameriturf Twin Oaks Environmental & Noble Turf | 403549 | 39 | 30 | Interstate |
| Dawson Truck Lines INC | 1928473 | 36 | 30 | Interstate |
| Chem-Solv INC | 349234 | 30 | 60 | InterstateHazmat |
| Fat Boys Wrecker Service INC | 3228705 | 30 | 10 | InterstateHazmat |
| Dehaven Transportation Company INC | 675970 | 28 | 23 | Interstate |
| Sams Transport LLC | 2779266 | 27 | 25 | Interstate |
| Mmc Transport Of Virginia | 374973 | 26 | 15 | Interstate |
| Summs Skip And Collateral Solutions | 587387 | 25 | 13 | InterstateHazmat |
| V F P INC | 4737 | 24 | 18 | Interstate |
| Virginia Transformer Corp | 842694 | 24 | 30 | Interstate |
| Goodwill Industries Of The Valleys | 1048165 | 24 | 20 | Interstate |
| Gregory Pallet And Lumber Company LLC | 780273 | 22 | 15 | Interstate |
| Weed Control | 1023994 | 20 | 30 | Interstate |
| P A Short Distributing CO | 2254266 | 20 | 14 | Interstate |
| Cii Service Of Southwest Virginia INC | 4147689 | 17 | 5 | Interstate |
| Award Express LLC | 2901431 | 16 | 17 | Interstate |
| Webb's Oil Corporation | 173291 | 13 | 9 | InterstateHazmat |
| US Crane And Rigging INC | 2372256 | 13 | 20 | Intrastate |
| Chins Transport INC | 2820885 | 13 | 13 | Interstate |
| Virginia Truck Center INC | 343336 | 12 | 20 | InterstateHazmat |
| Star City Games Com | 2431034 | 12 | 2 | Interstate |
| Hutch Services INC | 4340436 | 12 | 10 | Interstate |
| Cmc Supply INC | 363917 | 11 | 13 | InterstateHazmat |
| Virginia Varsity Transfer INC | 607292 | 11 | 13 | Interstate |
| Croy Contracting INC | 3742875 | 11 | 8 | Interstate |
| Ds Smith Packaging Roanoke | 61608 | 10 | 7 | Interstate |
| Roanoke Oil Distributors | 280592 | 10 | 8 | IntrastateHazmat |
| E C Pace Company INC | 544139 | 10 | 12 | Intrastate |
| Prestige Limousine INC | 820193 | 10 | 6 | Intrastate |
| Gap Logistics LLC | 3490569 | 10 | 10 | Interstate |
| Ech Trucking | 3725429 | 10 | 9 | Interstate |
| Ecc Contracting LLC | 4197359 | 10 | 4 | Interstate |
| Security Transport And Delivery Service INC | 84366 | 9 | 7 | Interstate |
| Roanoke Fruit & Produce Company | 37705 | 8 | 8 | Intrastate |
| Hollins Exposition Services | 418557 | 8 | 4 | Interstate |
| General Sales Of Va | 482212 | 8 | 6 | Interstate |
| Joe Bandy & Son INC | 1334049 | 8 | 5 | IntrastateHazmat |
| Crowe INC | 1657136 | 8 | 8 | Intrastate |
| Lawson Logistics INC | 1726666 | 8 | 8 | Interstate |
| Aaron's Sales And Lease Ownership | 1811215 | 8 | 6 | Intrastate |
| Gamstar Trucking LLC | 3438582 | 8 | 7 | Interstate |
| Rosel Transportation LLC | 3449602 | 8 | 8 | Interstate |
| Dream Transport LLC | 3839065 | 8 | 8 | Interstate |
| Mitchell's Towing And Recovery LLC | 4065410 | 8 | 40 | InterstateHazmat |
| Clean Valley Environmental Services LLC | 2204907 | 7 | 7 | Interstate |
| Budget Signs | 2910985 | 7 | 5 | Interstate |
| Ace Repair & Towing LLC | 3048097 | 7 | 5 | IntrastateHazmat |
| Kinsey Crane & Sign CO INC | 465749 | 6 | 7 | Intrastate |
| Time Technologies INC | 817763 | 6 | 6 | Interstate |
| National Pools Of Roanoke INC | 1815955 | 6 | 5 | InterstateHazmat |
| F & S Building Innovations INC | 2496343 | 6 | 3 | Intrastate |
| Bobcat Of Roanoke Valley | 2515026 | 6 | 3 | Interstate |
| Ace Steel International Corporation | 3382594 | 6 | 3 | Intrastate |
| A And D Towing | 3781778 | 6 | 5 | IntrastateHazmat |
| Star Valley Provisions | 4211456 | 6 | 5 | Interstate |
| Southern Refrigeration Corporation | 406757 | 5 | 14 | InterstateHazmat |
| Dutt & Wagner Of Va INC | 418863 | 5 | 5 | Intrastate |
| G J Hopkins INC | 468871 | 5 | 3 | InterstateHazmat |
| Virginia Scrap Iron And Metal Company | 1365474 | 5 | 1 | Intrastate |
| Solution 65 | 3089901 | 5 | 2 | Interstate |
| Ssm Transport LLC | 3514109 | 5 | 5 | Interstate |
| One Stop Automotive Sales & Service | 3771070 | 5 | 4 | InterstateHazmat |
| Chris Transportations LLC | 4116900 | 5 | 2 | Interstate |
| Bakr Logistics LLC | 4119245 | 5 | 4 | Interstate |
| Bakr Logistics LLC | 8862106 | 5 | 12 | Intrastate |
| Hales Landscaping INC | 397088 | 4 | 4 | Intrastate |
| Stage Sound INC | 421722 | 4 | 4 | Interstate |
| Dominion Air & Machinery CO | 456811 | 4 | 2 | Interstate |
| Barrows | 520164 | 4 | 5 | Interstate |
| Air Freight Expeditors | 883305 | 4 | 3 | InterstateHazmat |
| Cave Spring Construction INC | 1187067 | 4 | 4 | Intrastate |
| D L Barker Paving | 1347020 | 4 | 2 | Interstate |
| Amani | 2058585 | 4 | 4 | Interstate |
| Bds Holdings INC | 2415808 | 4 | 6 | Interstate |
| Southern Comfort INC | 2462690 | 4 | 4 | Intrastate |
| Central Virginia Well Fracturing | 2573546 | 4 | 8 | InterstateHazmat |
| Kvm Trucking LLC | 3369798 | 4 | 4 | Interstate |
| Nalamai LLC | 3790262 | 4 | 4 | Interstate |
| Ace Moving And Storage | 6113857 | 4 | 16 | Interstate |
| Medical Facilities Of America Administrative Consulting Services LLC | 8127852 | 4 | 4 | Intrastate |
| Roanoke Roofing & Sheet Metal CO INC | 375158 | 3 | 4 | Interstate |
| Hazzards Excavating & Trucking CO | 853113 | 3 | 2 | Intrastate |
| Collegiate Movers INC | 861003 | 3 | 3 | Interstate |
| Crowning Touch Senior Moving Services | 876104 | 3 | 9 | InterstateHazmat |
| Executive Town Car & Limousine Service INC | 1019939 | 3 | 15 | Interstate |
| R & T Lynch Transport & Logistics LLC | 1300088 | 3 | 3 | Interstate |
| Air Mechanical Solutions | 1391685 | 3 | 3 | Interstate |
| Masonry Mart | 1407135 | 3 | 7 | Intrastate |